The Hybrid Protein of the Alkaline Sensor IRR and the Fluorescent Protein GFP Retains the Functional Activity of the Receptor
- Authors: Mozhaev A.A.1,2, Serova O.V.1, Orsa A.N.1, Boyko A.A.1, Goryashchenko A.S.1, Deyev I.E.1, Petrenko A.G.1
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Affiliations:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Shubnikov Institute of Crystallography, Crystallography and Photonics Research Center, Russian Academy of Sciences
- Issue: Vol 45, No 2 (2019)
- Pages: 179-182
- Section: Letter to Editor
- URL: https://journals.rcsi.science/1068-1620/article/view/229187
- DOI: https://doi.org/10.1134/S1068162019020080
- ID: 229187
Cite item
Abstract
The insulin receptor-related receptor (IRR) is a cellular sensor of a weakly alkaline medium. Its spatial structure and the mechanism of activation have not been yet established. In the present work, a system of heterologous expression of full-length IRR tyrosine kinase has been created. A plasmid construct encoding the cDNA of the full-length human IRR fused at the C-terminus with the green fluorescent protein (GFP) has been generated, and the hybrid protein has been expressed in HEK293 line cells. It has been shown that the receptor incorporated in the hybrid protein is expressed on the cytoplasmic membrane and retains its functional activity. The resulting protein can be used in further studies of the structure and function of IRR.
About the authors
A. A. Mozhaev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Shubnikov Institute of Crystallography, Crystallography and Photonics Research Center, Russian Academy of Sciences
Email: petrenkoag@gmail.com
Russian Federation, Moscow, 117997; Moscow, 119333
O. V. Serova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: petrenkoag@gmail.com
Russian Federation, Moscow, 117997
A. N. Orsa
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: petrenkoag@gmail.com
Russian Federation, Moscow, 117997
A. A. Boyko
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: petrenkoag@gmail.com
Russian Federation, Moscow, 117997
A. S. Goryashchenko
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: petrenkoag@gmail.com
Russian Federation, Moscow, 117997
I. E. Deyev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: petrenkoag@gmail.com
Russian Federation, Moscow, 117997
A. G. Petrenko
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Author for correspondence.
Email: petrenkoag@gmail.com
Russian Federation, Moscow, 117997