Synthesis of a New Vitamin D 3  Hapten and Its Protein Conjugates

R. P. Litvinovskaya; A. P. Savachka; A. L. Sauchuk; A. G. Pradko; T. V. Mirantsova; V. A. Khripach

doi:10.1134/S1068162018050102

Synthesis of a New Vitamin D₃ Hapten and Its Protein Conjugates

作者: Litvinovskaya R.¹, Savachka A.¹, Sauchuk A.¹, Pradko A.², Mirantsova T.², Khripach V.¹
隶属关系:
1. Institute of Bioorganic Chemistry
2. Hemma-Test Ltd.
期: 卷 44, 编号 6 (2018)
页面: 778-786
栏目: Article
URL: https://journals.rcsi.science/1068-1620/article/view/229066
DOI: https://doi.org/10.1134/S1068162018050102
ID: 229066

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The synthesis of 25-(1-carboxymethoxy)-imino-3(S)-hydroxy-9,10-seco-27-norcholesta-5(Z),7(E),10(19)-triene, a new hapten of vitamin D₃, has been carried out in ten steps from ergosterol. The hapten was conjugated to horseradish peroxidase and bovine serum albumin. Antibodies to 25-hydroxyvitamin D₃ were obtained using the synthesized conjugates. An immunochemical system was developed for the quantitative determination of 25-hydroxyvitamin D₃.