Synthesis of a New Vitamin D3 Hapten and Its Protein Conjugates
- Autores: Litvinovskaya R.1, Savachka A.1, Sauchuk A.1, Pradko A.2, Mirantsova T.2, Khripach V.1
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Afiliações:
- Institute of Bioorganic Chemistry
- Hemma-Test Ltd.
- Edição: Volume 44, Nº 6 (2018)
- Páginas: 778-786
- Seção: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229066
- DOI: https://doi.org/10.1134/S1068162018050102
- ID: 229066
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Resumo
The synthesis of 25-(1-carboxymethoxy)-imino-3(S)-hydroxy-9,10-seco-27-norcholesta-5(Z),7(E),10(19)-triene, a new hapten of vitamin D3, has been carried out in ten steps from ergosterol. The hapten was conjugated to horseradish peroxidase and bovine serum albumin. Antibodies to 25-hydroxyvitamin D3 were obtained using the synthesized conjugates. An immunochemical system was developed for the quantitative determination of 25-hydroxyvitamin D3.
Sobre autores
R. Litvinovskaya
Institute of Bioorganic Chemistry
Autor responsável pela correspondência
Email: litvin@iboch.by
Belarus, Minsk, 220141
A. Savachka
Institute of Bioorganic Chemistry
Email: litvin@iboch.by
Belarus, Minsk, 220141
A. Sauchuk
Institute of Bioorganic Chemistry
Email: litvin@iboch.by
Belarus, Minsk, 220141
A. Pradko
Hemma-Test Ltd.
Email: litvin@iboch.by
Belarus, Minsk, 220029
T. Mirantsova
Hemma-Test Ltd.
Email: litvin@iboch.by
Belarus, Minsk, 220029
V. Khripach
Institute of Bioorganic Chemistry
Email: litvin@iboch.by
Belarus, Minsk, 220141