Enviar artigo por via de e-mail Detection of staphylococcal enterotoxin a by phage display mediated immuno-PCR method
- Autores: Artykov A.1, Fursova K.2,1, Ryazantsev D.3, Shchannikova M.2,1, Loskutova I.2,1, Shepelyakovskaya A.2,1, Laman A.1, Zavriev S.3, Brovko F.2,1
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Afiliações:
- Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Ernst All-Russia Research Institute of Animal Husbandry
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Edição: Volume 43, Nº 5 (2017)
- Páginas: 540-543
- Seção: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228656
- DOI: https://doi.org/10.1134/S1068162017050028
- ID: 228656
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Resumo
A method of staphylococcal enterotoxin A (SEA) detection in pasteurized milk using recombinant miniantibodies exposed on phage particles was developed. Miniantibodies bind to the antigen, while phage DNA serves as a matrix in polymerase chain reaction (PCR). The SEA detection limit in milk by a phage display mediated immuno-PCR method is 100 pg/mL that is much lower than the level of toxic and allergic dose.
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Sobre autores
A. Artykov
Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Rússia, Pushchino, Moscow oblast, 142290
K. Fursova
Ernst All-Russia Research Institute of Animal Husbandry; Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Rússia, Dubrovitsy, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
D. Ryazantsev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Rússia, Moscow, 117997
M. Shchannikova
Ernst All-Russia Research Institute of Animal Husbandry; Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Rússia, Dubrovitsy, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
I. Loskutova
Ernst All-Russia Research Institute of Animal Husbandry; Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Rússia, Dubrovitsy, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
A. Shepelyakovskaya
Ernst All-Russia Research Institute of Animal Husbandry; Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Rússia, Dubrovitsy, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
A. Laman
Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Rússia, Pushchino, Moscow oblast, 142290
S. Zavriev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Rússia, Moscow, 117997
F. Brovko
Ernst All-Russia Research Institute of Animal Husbandry; Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Autor responsável pela correspondência
Email: brovko@bibch.ru
Rússia, Dubrovitsy, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
