Email this article Detection of staphylococcal enterotoxin a by phage display mediated immuno-PCR method
- Authors: Artykov A.A.1, Fursova K.K.2,1, Ryazantsev D.Y.3, Shchannikova M.P.2,1, Loskutova I.V.2,1, Shepelyakovskaya A.O.2,1, Laman A.G.1, Zavriev S.K.3, Brovko F.A.2,1
-
Affiliations:
- Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Ernst All-Russia Research Institute of Animal Husbandry
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Issue: Vol 43, No 5 (2017)
- Pages: 540-543
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228656
- DOI: https://doi.org/10.1134/S1068162017050028
- ID: 228656
Cite item
Open Access
Access granted
Subscription Access
Abstract
A method of staphylococcal enterotoxin A (SEA) detection in pasteurized milk using recombinant miniantibodies exposed on phage particles was developed. Miniantibodies bind to the antigen, while phage DNA serves as a matrix in polymerase chain reaction (PCR). The SEA detection limit in milk by a phage display mediated immuno-PCR method is 100 pg/mL that is much lower than the level of toxic and allergic dose.
About the authors
A. A. Artykov
Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Russian Federation, Pushchino, Moscow oblast, 142290
K. K. Fursova
Ernst All-Russia Research Institute of Animal Husbandry; Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Russian Federation, Dubrovitsy, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
D. Yu. Ryazantsev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Russian Federation, Moscow, 117997
M. P. Shchannikova
Ernst All-Russia Research Institute of Animal Husbandry; Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Russian Federation, Dubrovitsy, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
I. V. Loskutova
Ernst All-Russia Research Institute of Animal Husbandry; Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Russian Federation, Dubrovitsy, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
A. O. Shepelyakovskaya
Ernst All-Russia Research Institute of Animal Husbandry; Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Russian Federation, Dubrovitsy, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
A. G. Laman
Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Russian Federation, Pushchino, Moscow oblast, 142290
S. K. Zavriev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: brovko@bibch.ru
Russian Federation, Moscow, 117997
F. A. Brovko
Ernst All-Russia Research Institute of Animal Husbandry; Branch of the Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Author for correspondence.
Email: brovko@bibch.ru
Russian Federation, Dubrovitsy, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
