Enviar artigo por via de e-mail Staphylococcal enterotoxin a detection with phage displayed antibodies
- Autores: Fursova K.1,2, Shchannikova M.1,2, Shepelyakovskaya A.1,2, Pavlik L.3, Brovko F.1,2
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Afiliações:
- Ernst Institute of Animal Husbandry
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry (Pushchino Branch)
- Institute of Theoretical and Experimental Biophysics
- Edição: Volume 42, Nº 3 (2016)
- Páginas: 294-299
- Seção: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/227931
- DOI: https://doi.org/10.1134/S1068162016020035
- ID: 227931
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Resumo
A technique for detection of staphylococcal enterotoxin A with sandwich enzyme-linked immunoassay (ELISA) was developed. Mouse monoclonal anti-SEA antibodies were used as capture antibodies and phage displayed anti-SEA scFv were used as detection antibodies. The limit of detection was 6–12.5 ng/mL for different pairs of antibodies. Some conditions of phage-displayed antibodies for storage in dissolved and lyophilized state were examined. It was shown the use of trehalose and arginine preserved the functional activity of phage-displayed antibodies.
Sobre autores
K. Fursova
Ernst Institute of Animal Husbandry; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry (Pushchino Branch)
Email: brovko@bibch.ru
Rússia, Podolsk District, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
M. Shchannikova
Ernst Institute of Animal Husbandry; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry (Pushchino Branch)
Email: brovko@bibch.ru
Rússia, Podolsk District, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
A. Shepelyakovskaya
Ernst Institute of Animal Husbandry; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry (Pushchino Branch)
Email: brovko@bibch.ru
Rússia, Podolsk District, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
L. Pavlik
Institute of Theoretical and Experimental Biophysics
Email: brovko@bibch.ru
Rússia, Pushchino, Moscow oblast, 142290
F. Brovko
Ernst Institute of Animal Husbandry; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry (Pushchino Branch)
Autor responsável pela correspondência
Email: brovko@bibch.ru
Rússia, Podolsk District, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
