Email this article Staphylococcal enterotoxin a detection with phage displayed antibodies
- Authors: Fursova K.K.1,2, Shchannikova M.P.1,2, Shepelyakovskaya A.O.1,2, Pavlik L.L.3, Brovko F.A.1,2
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Affiliations:
- Ernst Institute of Animal Husbandry
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry (Pushchino Branch)
- Institute of Theoretical and Experimental Biophysics
- Issue: Vol 42, No 3 (2016)
- Pages: 294-299
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/227931
- DOI: https://doi.org/10.1134/S1068162016020035
- ID: 227931
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Abstract
A technique for detection of staphylococcal enterotoxin A with sandwich enzyme-linked immunoassay (ELISA) was developed. Mouse monoclonal anti-SEA antibodies were used as capture antibodies and phage displayed anti-SEA scFv were used as detection antibodies. The limit of detection was 6–12.5 ng/mL for different pairs of antibodies. Some conditions of phage-displayed antibodies for storage in dissolved and lyophilized state were examined. It was shown the use of trehalose and arginine preserved the functional activity of phage-displayed antibodies.
About the authors
K. K. Fursova
Ernst Institute of Animal Husbandry; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry (Pushchino Branch)
Email: brovko@bibch.ru
Russian Federation, Podolsk District, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
M. P. Shchannikova
Ernst Institute of Animal Husbandry; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry (Pushchino Branch)
Email: brovko@bibch.ru
Russian Federation, Podolsk District, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
A. O. Shepelyakovskaya
Ernst Institute of Animal Husbandry; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry (Pushchino Branch)
Email: brovko@bibch.ru
Russian Federation, Podolsk District, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
L. L. Pavlik
Institute of Theoretical and Experimental Biophysics
Email: brovko@bibch.ru
Russian Federation, Pushchino, Moscow oblast, 142290
F. A. Brovko
Ernst Institute of Animal Husbandry; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry (Pushchino Branch)
Author for correspondence.
Email: brovko@bibch.ru
Russian Federation, Podolsk District, Moscow oblast, 142132; Pushchino, Moscow oblast, 142290
