Generation and Characterization of a Neutralizing Monoclonal Antibody Against Rabies Virus
- 作者: Ilina E.N.1, Solopova O.N.2,3, Balabashin D.S.4, Larina M.V.4, Aliev T.K.5, Grebennikova T.V.6, Losich M.A.6, Zaykova O.N.6, Sveshnikov P.G.2, Dolgikh D.A.1,4, Kirpichnikov M.P.1,4
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隶属关系:
- Biology Faculty
- All-Russia Research Center for Molecular Diagnostics and Treatment
- Blokhin National Medical Research Oncology Center
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
- Chemistry Faculty
- Gamaleya National Research Center for Epidemiology and Microbiology
- 期: 卷 44, 编号 6 (2018)
- 页面: 695-704
- 栏目: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229131
- DOI: https://doi.org/10.1134/S1068162019010072
- ID: 229131
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详细
Rabies is a zoonotic disease, for which effective treatment methods after the onset of clinical symptoms have not been developed yet. Polyclonal sera, both human and equine, along with vaccines are important means of disease prophylaxis. However, due to adverse reactions to the immunoglobulins of animal origin, high cost, and limited availability of the safer human serum, polyclonal antibodies should be substituted for a stable and efficient preparation, which is recombinant neutralizing antirabies monoclonal antibodies (mAbs). This paper reports generation of the humanized mAb 1C5, which binds with the antigenic site (AS) III of the rabies virus glycoprotein (RABVG) and demonstrates high virus neutralization activity in the fluorescent antibody virus neutralization test, as a result of expression in the Chinese hamster ovary (CHO) cells.
作者简介
E. Ilina
Biology Faculty
编辑信件的主要联系方式.
Email: Ekaterina.iljina.7@yandex.ru
俄罗斯联邦, Moscow, 119991
O. Solopova
All-Russia Research Center for Molecular Diagnostics and Treatment; Blokhin National Medical Research Oncology Center
Email: Ekaterina.iljina.7@yandex.ru
俄罗斯联邦, Moscow, 117149; Moscow, 115478
D. Balabashin
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: Ekaterina.iljina.7@yandex.ru
俄罗斯联邦, Moscow, 117997
M. Larina
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: Ekaterina.iljina.7@yandex.ru
俄罗斯联邦, Moscow, 117997
T. Aliev
Chemistry Faculty
Email: Ekaterina.iljina.7@yandex.ru
俄罗斯联邦, Moscow, 119991
T. Grebennikova
Gamaleya National Research Center for Epidemiology and Microbiology
Email: Ekaterina.iljina.7@yandex.ru
俄罗斯联邦, Moscow, 123098
M. Losich
Gamaleya National Research Center for Epidemiology and Microbiology
Email: Ekaterina.iljina.7@yandex.ru
俄罗斯联邦, Moscow, 123098
O. Zaykova
Gamaleya National Research Center for Epidemiology and Microbiology
Email: Ekaterina.iljina.7@yandex.ru
俄罗斯联邦, Moscow, 123098
P. Sveshnikov
All-Russia Research Center for Molecular Diagnostics and Treatment
Email: Ekaterina.iljina.7@yandex.ru
俄罗斯联邦, Moscow, 117149
D. Dolgikh
Biology Faculty; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: Ekaterina.iljina.7@yandex.ru
俄罗斯联邦, Moscow, 119991; Moscow, 117997
M. Kirpichnikov
Biology Faculty; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: Ekaterina.iljina.7@yandex.ru
俄罗斯联邦, Moscow, 119991; Moscow, 117997
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