Generation and Characterization of a Neutralizing Monoclonal Antibody Against Rabies Virus


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Abstract

Rabies is a zoonotic disease, for which effective treatment methods after the onset of clinical symptoms have not been developed yet. Polyclonal sera, both human and equine, along with vaccines are important means of disease prophylaxis. However, due to adverse reactions to the immunoglobulins of animal origin, high cost, and limited availability of the safer human serum, polyclonal antibodies should be substituted for a stable and efficient preparation, which is recombinant neutralizing antirabies monoclonal antibodies (mAbs). This paper reports generation of the humanized mAb 1C5, which binds with the antigenic site (AS) III of the rabies virus glycoprotein (RABVG) and demonstrates high virus neutralization activity in the fluorescent antibody virus neutralization test, as a result of expression in the Chinese hamster ovary (CHO) cells.

About the authors

E. N. Ilina

Biology Faculty

Author for correspondence.
Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 119991

O. N. Solopova

All-Russia Research Center for Molecular Diagnostics and Treatment; Blokhin National Medical Research Oncology Center

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 117149; Moscow, 115478

D. S. Balabashin

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 117997

M. V. Larina

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 117997

T. K. Aliev

Chemistry Faculty

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 119991

T. V. Grebennikova

Gamaleya National Research Center for Epidemiology and Microbiology

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 123098

M. A. Losich

Gamaleya National Research Center for Epidemiology and Microbiology

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 123098

O. N. Zaykova

Gamaleya National Research Center for Epidemiology and Microbiology

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 123098

P. G. Sveshnikov

All-Russia Research Center for Molecular Diagnostics and Treatment

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 117149

D. A. Dolgikh

Biology Faculty; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 119991; Moscow, 117997

M. P. Kirpichnikov

Biology Faculty; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 119991; Moscow, 117997

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