Generation and Characterization of a Neutralizing Monoclonal Antibody Against Rabies Virus
- Authors: Ilina E.N.1, Solopova O.N.2,3, Balabashin D.S.4, Larina M.V.4, Aliev T.K.5, Grebennikova T.V.6, Losich M.A.6, Zaykova O.N.6, Sveshnikov P.G.2, Dolgikh D.A.1,4, Kirpichnikov M.P.1,4
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Affiliations:
- Biology Faculty
- All-Russia Research Center for Molecular Diagnostics and Treatment
- Blokhin National Medical Research Oncology Center
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
- Chemistry Faculty
- Gamaleya National Research Center for Epidemiology and Microbiology
- Issue: Vol 44, No 6 (2018)
- Pages: 695-704
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229131
- DOI: https://doi.org/10.1134/S1068162019010072
- ID: 229131
Cite item
Abstract
Rabies is a zoonotic disease, for which effective treatment methods after the onset of clinical symptoms have not been developed yet. Polyclonal sera, both human and equine, along with vaccines are important means of disease prophylaxis. However, due to adverse reactions to the immunoglobulins of animal origin, high cost, and limited availability of the safer human serum, polyclonal antibodies should be substituted for a stable and efficient preparation, which is recombinant neutralizing antirabies monoclonal antibodies (mAbs). This paper reports generation of the humanized mAb 1C5, which binds with the antigenic site (AS) III of the rabies virus glycoprotein (RABVG) and demonstrates high virus neutralization activity in the fluorescent antibody virus neutralization test, as a result of expression in the Chinese hamster ovary (CHO) cells.
About the authors
E. N. Ilina
Biology Faculty
Author for correspondence.
Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 119991
O. N. Solopova
All-Russia Research Center for Molecular Diagnostics and Treatment; Blokhin National Medical Research Oncology Center
Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 117149; Moscow, 115478
D. S. Balabashin
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 117997
M. V. Larina
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 117997
T. K. Aliev
Chemistry Faculty
Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 119991
T. V. Grebennikova
Gamaleya National Research Center for Epidemiology and Microbiology
Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 123098
M. A. Losich
Gamaleya National Research Center for Epidemiology and Microbiology
Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 123098
O. N. Zaykova
Gamaleya National Research Center for Epidemiology and Microbiology
Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 123098
P. G. Sveshnikov
All-Russia Research Center for Molecular Diagnostics and Treatment
Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 117149
D. A. Dolgikh
Biology Faculty; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 119991; Moscow, 117997
M. P. Kirpichnikov
Biology Faculty; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow, 119991; Moscow, 117997
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