Optimization of the Technology for the Preparation of Cationic Liposomes for the Delivery of Nucleic Acids
- Authors: Luneva A.S.1, Puchkov P.A.1, Shmendel E.V.1, Zenkova M.A.2, Kuzevanova A.Y.3, Alimov A.A.3, Karpukhin A.V.3, Maslov M.A.1
-
Affiliations:
- Lomonosov Institute of Fine Chemical Technologies
- Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
- Research Center for Medical Genetics
- Issue: Vol 44, No 6 (2018)
- Pages: 724-731
- Section: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/229139
- DOI: https://doi.org/10.1134/S1068162019010084
- ID: 229139
Cite item
Abstract
Cationic liposomes based on the cationic lipid 1,26-bis(cholest-5-en-3β-yloxycarbonylamino)- 7,11,16-2-tetraazahexacosane tetrahydrochloride (2X3) and the helper lipid 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) have been prepared by two methods, namely ultrasonication and the extrusion of a lipid dispersion. The effect of the preparation method and the type of the water phase on the physicochemical characteristics of cationic liposomes and their biological activity has been studied. The optimization of the preparation of cationic liposomes made it possible to determine the technological parameters providing the reproducibility of physicochemical characteristics of liposomes from batch to batch.
About the authors
A. S. Luneva
Lomonosov Institute of Fine Chemical Technologies
Email: mamaslov@mail.ru
Russian Federation, Moscow, 119571
P. A. Puchkov
Lomonosov Institute of Fine Chemical Technologies
Email: mamaslov@mail.ru
Russian Federation, Moscow, 119571
E. V. Shmendel
Lomonosov Institute of Fine Chemical Technologies
Email: mamaslov@mail.ru
Russian Federation, Moscow, 119571
M. A. Zenkova
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
Email: mamaslov@mail.ru
Russian Federation, Novosibirsk, 630090
A. Yu. Kuzevanova
Research Center for Medical Genetics
Email: mamaslov@mail.ru
Russian Federation, Moscow, 115478
A. A. Alimov
Research Center for Medical Genetics
Email: mamaslov@mail.ru
Russian Federation, Moscow, 115478
A. V. Karpukhin
Research Center for Medical Genetics
Email: mamaslov@mail.ru
Russian Federation, Moscow, 115478
M. A. Maslov
Lomonosov Institute of Fine Chemical Technologies
Author for correspondence.
Email: mamaslov@mail.ru
Russian Federation, Moscow, 119571