A synthesis of tritium-labeled juvenile hormone and radiometric analysis of the enzymatic hydrolysis level

I. V. Romanova; A. A. Alekseev; V. A. Richter; N. E. Gruntenko; A. M. Agafontsev; E. K. Karpova

doi:10.1134/S1068162017020121

A synthesis of tritium-labeled juvenile hormone and radiometric analysis of the enzymatic hydrolysis level

Authors: Romanova I.V.¹, Alekseev A.A.², Richter V.A.¹, Gruntenko N.E.³, Agafontsev A.M.⁴, Karpova E.K.³
Affiliations:
1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
2. Voevodsky Institute of Chemical Kinetics and Combustion, Siberian Branch
3. Institute of Cytology and Genetics, Siberian Branch
4. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch
Issue: Vol 43, No 2 (2017)
Pages: 211-215
Section: Article
URL: https://journals.rcsi.science/1068-1620/article/view/228489
DOI: https://doi.org/10.1134/S1068162017020121
ID: 228489

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Abstract

A method of insect juvenile hormone III (JH-III) labeling by thermally activated tritium was developed. Purification of the labeled compound was performed on a TLC silica gel plate using a system of hexane-ethyl acetate 2: 1. The molar radioactivity of [³H]JH-III was 2.2 Ci mmol^–1. It was shown that tritium-labeled juvenile hormone can be used in the radiometric method for quantitative measurement of the activity of specific enzymes that hydrolyze juvenile hormone.