A synthesis of tritium-labeled juvenile hormone and radiometric analysis of the enzymatic hydrolysis level
- 作者: Romanova I.1, Alekseev A.2, Richter V.1, Gruntenko N.3, Agafontsev A.4, Karpova E.3
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隶属关系:
- Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
- Voevodsky Institute of Chemical Kinetics and Combustion, Siberian Branch
- Institute of Cytology and Genetics, Siberian Branch
- Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch
- 期: 卷 43, 编号 2 (2017)
- 页面: 211-215
- 栏目: Article
- URL: https://journals.rcsi.science/1068-1620/article/view/228489
- DOI: https://doi.org/10.1134/S1068162017020121
- ID: 228489
如何引用文章
详细
A method of insect juvenile hormone III (JH-III) labeling by thermally activated tritium was developed. Purification of the labeled compound was performed on a TLC silica gel plate using a system of hexane-ethyl acetate 2: 1. The molar radioactivity of [3H]JH-III was 2.2 Ci mmol–1. It was shown that tritium-labeled juvenile hormone can be used in the radiometric method for quantitative measurement of the activity of specific enzymes that hydrolyze juvenile hormone.
作者简介
I. Romanova
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
Email: alekseev@kinetics.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
A. Alekseev
Voevodsky Institute of Chemical Kinetics and Combustion, Siberian Branch
编辑信件的主要联系方式.
Email: alekseev@kinetics.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
V. Richter
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
Email: alekseev@kinetics.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
N. Gruntenko
Institute of Cytology and Genetics, Siberian Branch
Email: alekseev@kinetics.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
A. Agafontsev
Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch
Email: alekseev@kinetics.nsc.ru
俄罗斯联邦, Novosibirsk, 630090
E. Karpova
Institute of Cytology and Genetics, Siberian Branch
Email: alekseev@kinetics.nsc.ru
俄罗斯联邦, Novosibirsk, 630090