Small-angle X-ray scattering study of conditions for the formation of growth units of protein crystals in lysozyme solutions
- Авторы: Dyakova Y.A.1,2, Ilina K.B.1,2, Konarev P.V.1,2, Kryukova A.E.1,2, Marchenkova M.A.1,2, Blagov A.E.1,2, Volkov V.V.1, Pisarevsky Y.V.1,2, Kovalchuk M.V.1,2,3
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Учреждения:
- Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”
- National Research Centre “Kurchatov Institute”
- St. Petersburg State University
- Выпуск: Том 62, № 3 (2017)
- Страницы: 364-369
- Раздел: Diffraction and Scattering of Ionizing Radiations
- URL: https://journals.rcsi.science/1063-7745/article/view/190953
- DOI: https://doi.org/10.1134/S1063774517030051
- ID: 190953
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Аннотация
The structural composition of lysozyme solutions favorable for the formation of the tetragonal form of protein crystals was studied by synchrotron-based small-angle X-ray scattering depending on the protein concentration and the temperature. Along with lysozyme monomers, dimers and octamers are found in crystallization solutions; the octamer content increases with an increase in the protein concentration.
Об авторах
Yu. Dyakova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”
Email: ilina-ks@mail.ru
Россия, Moscow, 119333; Moscow, 123098
K. Ilina
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”
Автор, ответственный за переписку.
Email: ilina-ks@mail.ru
Россия, Moscow, 119333; Moscow, 123098
P. Konarev
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”
Email: ilina-ks@mail.ru
Россия, Moscow, 119333; Moscow, 123098
A. Kryukova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”
Email: ilina-ks@mail.ru
Россия, Moscow, 119333; Moscow, 123098
M. Marchenkova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”
Email: ilina-ks@mail.ru
Россия, Moscow, 119333; Moscow, 123098
A. Blagov
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”
Email: ilina-ks@mail.ru
Россия, Moscow, 119333; Moscow, 123098
V. Volkov
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”
Email: ilina-ks@mail.ru
Россия, Moscow, 119333
Yu. Pisarevsky
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”
Email: ilina-ks@mail.ru
Россия, Moscow, 119333; Moscow, 123098
M. Kovalchuk
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”; St. Petersburg State University
Email: ilina-ks@mail.ru
Россия, Moscow, 119333; Moscow, 123098; St. Petersburg, 199034
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