Email this article Small-angle X-ray scattering study of conditions for the formation of growth units of protein crystals in lysozyme solutions
- Authors: Dyakova Y.A.1,2, Ilina K.B.1,2, Konarev P.V.1,2, Kryukova A.E.1,2, Marchenkova M.A.1,2, Blagov A.E.1,2, Volkov V.V.1, Pisarevsky Y.V.1,2, Kovalchuk M.V.1,2,3
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Affiliations:
- Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”
- National Research Centre “Kurchatov Institute”
- St. Petersburg State University
- Issue: Vol 62, No 3 (2017)
- Pages: 364-369
- Section: Diffraction and Scattering of Ionizing Radiations
- URL: https://journals.rcsi.science/1063-7745/article/view/190953
- DOI: https://doi.org/10.1134/S1063774517030051
- ID: 190953
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Abstract
The structural composition of lysozyme solutions favorable for the formation of the tetragonal form of protein crystals was studied by synchrotron-based small-angle X-ray scattering depending on the protein concentration and the temperature. Along with lysozyme monomers, dimers and octamers are found in crystallization solutions; the octamer content increases with an increase in the protein concentration.
About the authors
Yu. A. Dyakova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”
Email: ilina-ks@mail.ru
Russian Federation, Moscow, 119333; Moscow, 123098
K. B. Ilina
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”
Author for correspondence.
Email: ilina-ks@mail.ru
Russian Federation, Moscow, 119333; Moscow, 123098
P. V. Konarev
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”
Email: ilina-ks@mail.ru
Russian Federation, Moscow, 119333; Moscow, 123098
A. E. Kryukova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”
Email: ilina-ks@mail.ru
Russian Federation, Moscow, 119333; Moscow, 123098
M. A. Marchenkova
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”
Email: ilina-ks@mail.ru
Russian Federation, Moscow, 119333; Moscow, 123098
A. E. Blagov
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”
Email: ilina-ks@mail.ru
Russian Federation, Moscow, 119333; Moscow, 123098
V. V. Volkov
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”
Email: ilina-ks@mail.ru
Russian Federation, Moscow, 119333
Yu. V. Pisarevsky
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”
Email: ilina-ks@mail.ru
Russian Federation, Moscow, 119333; Moscow, 123098
M. V. Kovalchuk
Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”; St. Petersburg State University
Email: ilina-ks@mail.ru
Russian Federation, Moscow, 119333; Moscow, 123098; St. Petersburg, 199034
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