Small-angle X-ray scattering study of conditions for the formation of growth units of protein crystals in lysozyme solutions


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Resumo

The structural composition of lysozyme solutions favorable for the formation of the tetragonal form of protein crystals was studied by synchrotron-based small-angle X-ray scattering depending on the protein concentration and the temperature. Along with lysozyme monomers, dimers and octamers are found in crystallization solutions; the octamer content increases with an increase in the protein concentration.

Sobre autores

Yu. Dyakova

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”

Email: ilina-ks@mail.ru
Rússia, Moscow, 119333; Moscow, 123098

K. Ilina

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”

Autor responsável pela correspondência
Email: ilina-ks@mail.ru
Rússia, Moscow, 119333; Moscow, 123098

P. Konarev

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”

Email: ilina-ks@mail.ru
Rússia, Moscow, 119333; Moscow, 123098

A. Kryukova

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”

Email: ilina-ks@mail.ru
Rússia, Moscow, 119333; Moscow, 123098

M. Marchenkova

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”

Email: ilina-ks@mail.ru
Rússia, Moscow, 119333; Moscow, 123098

A. Blagov

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”

Email: ilina-ks@mail.ru
Rússia, Moscow, 119333; Moscow, 123098

V. Volkov

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”

Email: ilina-ks@mail.ru
Rússia, Moscow, 119333

Yu. Pisarevsky

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”

Email: ilina-ks@mail.ru
Rússia, Moscow, 119333; Moscow, 123098

M. Kovalchuk

Shubnikov Institute of Crystallography of Federal Scientific Research Centre “Crystallography and Photonics”; National Research Centre “Kurchatov Institute”; St. Petersburg State University

Email: ilina-ks@mail.ru
Rússia, Moscow, 119333; Moscow, 123098; St. Petersburg, 199034

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