Spectrophotometric determination of proteins in biological fluids
- 作者: Anisimovich P.1, Pochinok T.1, Tokareva E.1
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隶属关系:
- Faculty of Chemistry and High Technologies
- 期: 卷 72, 编号 12 (2017)
- 页面: 1212-1218
- 栏目: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182686
- DOI: https://doi.org/10.1134/S1061934817120024
- ID: 182686
如何引用文章
详细
A comparative analysis of procedures of the spectrophotometric determination of total protein with various organic dyes, Bromocresol Green, Bromophenol Blue, and Pyrogallol Red, in biological fluids is presented. It is shown that the results of determination with various dyes can differ because of the specific features of reagent interaction with the components of biological fluids. A new organic reagent, Bromopyrogallol Red, possessing equal sensitivity to different protein fractions (albumins and globulins) and ensuring a minimum error of the determination of total protein in clinical examinations using calibration solutions of various compositions is proposed. A procedure for the determination of total protein in biological fluids is developed and tested on real samples of urine and blood serum.
作者简介
P. Anisimovich
Faculty of Chemistry and High Technologies
Email: pochinokt@chem.kubsu.ru
俄罗斯联邦, Krasnodar, 350040
T. Pochinok
Faculty of Chemistry and High Technologies
编辑信件的主要联系方式.
Email: pochinokt@chem.kubsu.ru
俄罗斯联邦, Krasnodar, 350040
E. Tokareva
Faculty of Chemistry and High Technologies
Email: pochinokt@chem.kubsu.ru
俄罗斯联邦, Krasnodar, 350040
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