Spectrophotometric determination of proteins in biological fluids
- Авторы: Anisimovich P.1, Pochinok T.1, Tokareva E.1
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Учреждения:
- Faculty of Chemistry and High Technologies
- Выпуск: Том 72, № 12 (2017)
- Страницы: 1212-1218
- Раздел: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182686
- DOI: https://doi.org/10.1134/S1061934817120024
- ID: 182686
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Аннотация
A comparative analysis of procedures of the spectrophotometric determination of total protein with various organic dyes, Bromocresol Green, Bromophenol Blue, and Pyrogallol Red, in biological fluids is presented. It is shown that the results of determination with various dyes can differ because of the specific features of reagent interaction with the components of biological fluids. A new organic reagent, Bromopyrogallol Red, possessing equal sensitivity to different protein fractions (albumins and globulins) and ensuring a minimum error of the determination of total protein in clinical examinations using calibration solutions of various compositions is proposed. A procedure for the determination of total protein in biological fluids is developed and tested on real samples of urine and blood serum.
Об авторах
P. Anisimovich
Faculty of Chemistry and High Technologies
Email: pochinokt@chem.kubsu.ru
Россия, Krasnodar, 350040
T. Pochinok
Faculty of Chemistry and High Technologies
Автор, ответственный за переписку.
Email: pochinokt@chem.kubsu.ru
Россия, Krasnodar, 350040
E. Tokareva
Faculty of Chemistry and High Technologies
Email: pochinokt@chem.kubsu.ru
Россия, Krasnodar, 350040