An HPLC method for levofloxacin determination and its application in biomedical analysis
- 作者: Czyrski A.1, Szałek E.2
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隶属关系:
- Department of Physical Pharmacy and Pharmacokinetics
- Department of Clinical Pharmacy and Biopharmacy
- 期: 卷 71, 编号 8 (2016)
- 页面: 840-843
- 栏目: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181998
- DOI: https://doi.org/10.1134/S1061934816080049
- ID: 181998
如何引用文章
详细
Levofloxacin is a bacteriostatic L-isomer of ofloxacin. This drug is a third generation fluoroquinolone. Its activity strongly depends on its concentration and the AUC/MIC ratio is the best indication of drug exposure. A simple, specific and fast HPLC method with fluorescence detection for assay of levofloxacin level in human plasma was developed. The separation was performed on a reversed phase column. The mobile phase contained 0.4% solution of triethylamine. The regression equation was linear within the concentration range 0.15–30.0 mg/L. The validation parameters i.e. accuracy, precision and stability did not exceed 10%. High recovery was observed. The developed method fulfils the validation parameters. Due to the short time of analysis it may be applied to biomedical analysis.
作者简介
A. Czyrski
Department of Physical Pharmacy and Pharmacokinetics
编辑信件的主要联系方式.
Email: aczyrski@ump.edu.pl
波兰, Święcickiego 6 Street, Poznań, 60-781
E. Szałek
Department of Clinical Pharmacy and Biopharmacy
Email: aczyrski@ump.edu.pl
波兰, Marii Magdaleny 14 Street, Poznań, 61-861