An HPLC method for levofloxacin determination and its application in biomedical analysis
- Authors: Czyrski A.1, Szałek E.2
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Affiliations:
- Department of Physical Pharmacy and Pharmacokinetics
- Department of Clinical Pharmacy and Biopharmacy
- Issue: Vol 71, No 8 (2016)
- Pages: 840-843
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181998
- DOI: https://doi.org/10.1134/S1061934816080049
- ID: 181998
Cite item
Abstract
Levofloxacin is a bacteriostatic L-isomer of ofloxacin. This drug is a third generation fluoroquinolone. Its activity strongly depends on its concentration and the AUC/MIC ratio is the best indication of drug exposure. A simple, specific and fast HPLC method with fluorescence detection for assay of levofloxacin level in human plasma was developed. The separation was performed on a reversed phase column. The mobile phase contained 0.4% solution of triethylamine. The regression equation was linear within the concentration range 0.15–30.0 mg/L. The validation parameters i.e. accuracy, precision and stability did not exceed 10%. High recovery was observed. The developed method fulfils the validation parameters. Due to the short time of analysis it may be applied to biomedical analysis.
Keywords
About the authors
A. Czyrski
Department of Physical Pharmacy and Pharmacokinetics
Author for correspondence.
Email: aczyrski@ump.edu.pl
Poland, Święcickiego 6 Street, Poznań, 60-781
E. Szałek
Department of Clinical Pharmacy and Biopharmacy
Email: aczyrski@ump.edu.pl
Poland, Marii Magdaleny 14 Street, Poznań, 61-861