An HPLC method for levofloxacin determination and its application in biomedical analysis
- Autores: Czyrski A.1, Szałek E.2
-
Afiliações:
- Department of Physical Pharmacy and Pharmacokinetics
- Department of Clinical Pharmacy and Biopharmacy
- Edição: Volume 71, Nº 8 (2016)
- Páginas: 840-843
- Seção: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181998
- DOI: https://doi.org/10.1134/S1061934816080049
- ID: 181998
Citar
Resumo
Levofloxacin is a bacteriostatic L-isomer of ofloxacin. This drug is a third generation fluoroquinolone. Its activity strongly depends on its concentration and the AUC/MIC ratio is the best indication of drug exposure. A simple, specific and fast HPLC method with fluorescence detection for assay of levofloxacin level in human plasma was developed. The separation was performed on a reversed phase column. The mobile phase contained 0.4% solution of triethylamine. The regression equation was linear within the concentration range 0.15–30.0 mg/L. The validation parameters i.e. accuracy, precision and stability did not exceed 10%. High recovery was observed. The developed method fulfils the validation parameters. Due to the short time of analysis it may be applied to biomedical analysis.
Palavras-chave
Sobre autores
A. Czyrski
Department of Physical Pharmacy and Pharmacokinetics
Autor responsável pela correspondência
Email: aczyrski@ump.edu.pl
Polônia, Święcickiego 6 Street, Poznań, 60-781
E. Szałek
Department of Clinical Pharmacy and Biopharmacy
Email: aczyrski@ump.edu.pl
Polônia, Marii Magdaleny 14 Street, Poznań, 61-861