Determination of vitamin K1 in blood serum by high-performance liquid chromatography
- 作者: Sokolova O.1, Potapova I.1
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隶属关系:
- Nizhny Novgorod Research Institute of Hygiene and Occupational Pathology
- 期: 卷 71, 编号 7 (2016)
- 页面: 737-740
- 栏目: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181913
- DOI: https://doi.org/10.1134/S1061934816070157
- ID: 181913
如何引用文章
详细
A method is proposed for the determination of vitamin K1 concentration in blood serum using HPLC on a Gemini C18 (250 mm × 4.6 mm ID, 5 μm) column and elution with a methanol–acetonitrile–dichloromethane (45 : 50 : 5, v/v) mobile phase at a flow rate of 1 mL/min and detection at 248 nm. The limit of detection for the vitamin is 0.5 ng/mL and relative error is 19%. The volume of sample in the column is 100 μL. The method has been applied to the evaluation of vitamin K1 deficit in a human body in medical practice.
作者简介
O. Sokolova
Nizhny Novgorod Research Institute of Hygiene and Occupational Pathology
编辑信件的主要联系方式.
Email: ovs.work@yandex.ru
俄罗斯联邦, ul. Semashko 20, Nizhny Novgorod, 603950
I. Potapova
Nizhny Novgorod Research Institute of Hygiene and Occupational Pathology
Email: ovs.work@yandex.ru
俄罗斯联邦, ul. Semashko 20, Nizhny Novgorod, 603950
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