Email this article Determination of vitamin K1 in blood serum by high-performance liquid chromatography
- Authors: Sokolova O.V.1, Potapova I.A.1
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Affiliations:
- Nizhny Novgorod Research Institute of Hygiene and Occupational Pathology
- Issue: Vol 71, No 7 (2016)
- Pages: 737-740
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181913
- DOI: https://doi.org/10.1134/S1061934816070157
- ID: 181913
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Abstract
A method is proposed for the determination of vitamin K1 concentration in blood serum using HPLC on a Gemini C18 (250 mm × 4.6 mm ID, 5 μm) column and elution with a methanol–acetonitrile–dichloromethane (45 : 50 : 5, v/v) mobile phase at a flow rate of 1 mL/min and detection at 248 nm. The limit of detection for the vitamin is 0.5 ng/mL and relative error is 19%. The volume of sample in the column is 100 μL. The method has been applied to the evaluation of vitamin K1 deficit in a human body in medical practice.
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About the authors
O. V. Sokolova
Nizhny Novgorod Research Institute of Hygiene and Occupational Pathology
Author for correspondence.
Email: ovs.work@yandex.ru
Russian Federation, ul. Semashko 20, Nizhny Novgorod, 603950
I. A. Potapova
Nizhny Novgorod Research Institute of Hygiene and Occupational Pathology
Email: ovs.work@yandex.ru
Russian Federation, ul. Semashko 20, Nizhny Novgorod, 603950
