Spectrophotometric determination of proteins in biological fluids
- Authors: Anisimovich P.V.1, Pochinok T.B.1, Tokareva E.V.1
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Affiliations:
- Faculty of Chemistry and High Technologies
- Issue: Vol 72, No 12 (2017)
- Pages: 1212-1218
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182686
- DOI: https://doi.org/10.1134/S1061934817120024
- ID: 182686
Cite item
Abstract
A comparative analysis of procedures of the spectrophotometric determination of total protein with various organic dyes, Bromocresol Green, Bromophenol Blue, and Pyrogallol Red, in biological fluids is presented. It is shown that the results of determination with various dyes can differ because of the specific features of reagent interaction with the components of biological fluids. A new organic reagent, Bromopyrogallol Red, possessing equal sensitivity to different protein fractions (albumins and globulins) and ensuring a minimum error of the determination of total protein in clinical examinations using calibration solutions of various compositions is proposed. A procedure for the determination of total protein in biological fluids is developed and tested on real samples of urine and blood serum.
About the authors
P. V. Anisimovich
Faculty of Chemistry and High Technologies
Email: pochinokt@chem.kubsu.ru
Russian Federation, Krasnodar, 350040
T. B. Pochinok
Faculty of Chemistry and High Technologies
Author for correspondence.
Email: pochinokt@chem.kubsu.ru
Russian Federation, Krasnodar, 350040
E. V. Tokareva
Faculty of Chemistry and High Technologies
Email: pochinokt@chem.kubsu.ru
Russian Federation, Krasnodar, 350040