Determination of ecdysteroids in Fornicium unflorum (L.) and Serratula centauroides (L.) raw materials by chromatography–UV spectrophotometry
- Autores: Nikolaeva I.1,2, Tsybiktarova L.1,2, Garmaeva L.1, Nikolaeva G.1,2, Olennikov D.1, Matkhanov I.1
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Afiliações:
- Institute of General and Experimental Biology, Siberian Branch
- Buryat State University
- Edição: Volume 72, Nº 8 (2017)
- Páginas: 854-861
- Seção: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182589
- DOI: https://doi.org/10.1134/S1061934817080093
- ID: 182589
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Resumo
A chromatography–spectrophotometry method is developed for determining total ecdysteroids in terms of 20-hydroxyecdysone in Serratula centauroides (L.) and Fornicium unflorum (L.) raw materials, based on the purification of extracts from the plant raw materials with aluminum oxide and subsequent spectrophotometric determination relative to a 20-hydroxyecdysone reference sample. Ecdysteroids were determined in plants by HPLC; rhapontisterone and turkesterone are found in F. uniflorum for the first time.
Sobre autores
I. Nikolaeva
Institute of General and Experimental Biology, Siberian Branch; Buryat State University
Autor responsável pela correspondência
Email: i-nik@mail.ru
Rússia, Ulan-Ude, 670047; Ulan-Ude, 670000
L. Tsybiktarova
Institute of General and Experimental Biology, Siberian Branch; Buryat State University
Email: i-nik@mail.ru
Rússia, Ulan-Ude, 670047; Ulan-Ude, 670000
L. Garmaeva
Institute of General and Experimental Biology, Siberian Branch
Email: i-nik@mail.ru
Rússia, Ulan-Ude, 670047
G. Nikolaeva
Institute of General and Experimental Biology, Siberian Branch; Buryat State University
Email: i-nik@mail.ru
Rússia, Ulan-Ude, 670047; Ulan-Ude, 670000
D. Olennikov
Institute of General and Experimental Biology, Siberian Branch
Email: i-nik@mail.ru
Rússia, Ulan-Ude, 670047
I. Matkhanov
Institute of General and Experimental Biology, Siberian Branch
Email: i-nik@mail.ru
Rússia, Ulan-Ude, 670047
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