Determination of ecdysteroids in Fornicium unflorum (L.) and Serratula centauroides (L.) raw materials by chromatography–UV spectrophotometry
- Authors: Nikolaeva I.G.1,2, Tsybiktarova L.P.1,2, Garmaeva L.L.1, Nikolaeva G.G.1,2, Olennikov D.N.1, Matkhanov I.E.1
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Affiliations:
- Institute of General and Experimental Biology, Siberian Branch
- Buryat State University
- Issue: Vol 72, No 8 (2017)
- Pages: 854-861
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182589
- DOI: https://doi.org/10.1134/S1061934817080093
- ID: 182589
Cite item
Abstract
A chromatography–spectrophotometry method is developed for determining total ecdysteroids in terms of 20-hydroxyecdysone in Serratula centauroides (L.) and Fornicium unflorum (L.) raw materials, based on the purification of extracts from the plant raw materials with aluminum oxide and subsequent spectrophotometric determination relative to a 20-hydroxyecdysone reference sample. Ecdysteroids were determined in plants by HPLC; rhapontisterone and turkesterone are found in F. uniflorum for the first time.
About the authors
I. G. Nikolaeva
Institute of General and Experimental Biology, Siberian Branch; Buryat State University
Author for correspondence.
Email: i-nik@mail.ru
Russian Federation, Ulan-Ude, 670047; Ulan-Ude, 670000
L. P. Tsybiktarova
Institute of General and Experimental Biology, Siberian Branch; Buryat State University
Email: i-nik@mail.ru
Russian Federation, Ulan-Ude, 670047; Ulan-Ude, 670000
L. L. Garmaeva
Institute of General and Experimental Biology, Siberian Branch
Email: i-nik@mail.ru
Russian Federation, Ulan-Ude, 670047
G. G. Nikolaeva
Institute of General and Experimental Biology, Siberian Branch; Buryat State University
Email: i-nik@mail.ru
Russian Federation, Ulan-Ude, 670047; Ulan-Ude, 670000
D. N. Olennikov
Institute of General and Experimental Biology, Siberian Branch
Email: i-nik@mail.ru
Russian Federation, Ulan-Ude, 670047
I. E. Matkhanov
Institute of General and Experimental Biology, Siberian Branch
Email: i-nik@mail.ru
Russian Federation, Ulan-Ude, 670047
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