Comparison of separation performance of polysaccaride-based chiral stationary phases in enantioseparation of 1-(4-chlorobenzhydryl)piperazine benzamide derivatives by HPLC
- Autores: Gurdal E.1, Yarim M.1
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Afiliações:
- Department of Pharmaceutical Chemistry, Faculty of Pharmacy
- Edição: Volume 72, Nº 2 (2017)
- Páginas: 213-216
- Seção: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182310
- DOI: https://doi.org/10.1134/S1061934817020046
- ID: 182310
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Resumo
Analytical high-performance liquid chromatographic enantioseparation of 1-(4-chlorobenzhydryl) piperazine benzamide derivatives was accomplished on different chiral stationary phases. The enantiomers of the compounds were resolved by normal-phase chromatography on silica-based amylose tris(3,5-dimethylphenylcarbamate) (Chiralpak AD-H), cellulose tris(3,5-dimethylphenylcarbamate) (Chiralcel OD-H) and cellulose tris(4-methylbenzoate) (Chiralcel OJ) columns with mobile phases consisting of mixtures of n-hexane and ethanol in different proportions (90: 10, 80: 20). The mobile phase and the chiral stationary phase were varied to achieve the best resolution. The effect of the concentration of ethanol in the mobile phase was studied. The resolution obtained on the three columns was significant.
Sobre autores
Enise Gurdal
Department of Pharmaceutical Chemistry, Faculty of Pharmacy
Autor responsável pela correspondência
Email: egurdal@yeditepe.edu.tr
Turquia, Kayisdagi, Istanbul, 34755
Mine Yarim
Department of Pharmaceutical Chemistry, Faculty of Pharmacy
Email: egurdal@yeditepe.edu.tr
Turquia, Kayisdagi, Istanbul, 34755
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