Determination of Andarine (S-4), a Selective Androgen Receptor Modulator, and Ibutamoren (MK-677), a Nonpeptide Growth Hormone Secretagogue, in Urine by Ultra-High Performance Liquid Chromatography with Tandem Mass-Spectrometric Detection
- Authors: Dmitrieva E.V.1, Temerdashev A.Z.1, Azaryan A.A.1, Gashimova E.M.1
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Affiliations:
- Kuban State University
- Issue: Vol 73, No 7 (2018)
- Pages: 674-678
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183094
- DOI: https://doi.org/10.1134/S1061934818070031
- ID: 183094
Cite item
Abstract
A procedure for the determination of a selective androgen receptor modulator andarine (S-4) and a nonpeptide growth hormone secretagogue ibutamoren (MK-677) in urine has been developed including sample preparation by the “dilute-and-shoot” procedure, separation of analytes by ultra-high performance liquid chromatography in the gradient elution mode, and mass-spectrometric detection with heated electrospray ionization. The limits of detection are in the range of 0.5–2.5 ng/mL, the calibration curves are linear in the range of 2.5–250 ng/mL for andarine and 5–250 ng/mL for ibutamoren. The proposed procedure was used for the analysis of urine samples obtained from volunteers after a single administration of these drugs containing 15 mg of active substances.
About the authors
E. V. Dmitrieva
Kuban State University
Author for correspondence.
Email: catherine_dmitrieva@outlook.com
Russian Federation, Krasnodar, 350040
A. Z. Temerdashev
Kuban State University
Email: catherine_dmitrieva@outlook.com
Russian Federation, Krasnodar, 350040
A. A. Azaryan
Kuban State University
Email: catherine_dmitrieva@outlook.com
Russian Federation, Krasnodar, 350040
E. M. Gashimova
Kuban State University
Email: catherine_dmitrieva@outlook.com
Russian Federation, Krasnodar, 350040