Determination of Andarine (S-4), a Selective Androgen Receptor Modulator, and Ibutamoren (MK-677), a Nonpeptide Growth Hormone Secretagogue, in Urine by Ultra-High Performance Liquid Chromatography with Tandem Mass-Spectrometric Detection
- Autores: Dmitrieva E.1, Temerdashev A.1, Azaryan A.1, Gashimova E.1
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Afiliações:
- Kuban State University
- Edição: Volume 73, Nº 7 (2018)
- Páginas: 674-678
- Seção: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183094
- DOI: https://doi.org/10.1134/S1061934818070031
- ID: 183094
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Resumo
A procedure for the determination of a selective androgen receptor modulator andarine (S-4) and a nonpeptide growth hormone secretagogue ibutamoren (MK-677) in urine has been developed including sample preparation by the “dilute-and-shoot” procedure, separation of analytes by ultra-high performance liquid chromatography in the gradient elution mode, and mass-spectrometric detection with heated electrospray ionization. The limits of detection are in the range of 0.5–2.5 ng/mL, the calibration curves are linear in the range of 2.5–250 ng/mL for andarine and 5–250 ng/mL for ibutamoren. The proposed procedure was used for the analysis of urine samples obtained from volunteers after a single administration of these drugs containing 15 mg of active substances.
Sobre autores
E. Dmitrieva
Kuban State University
Autor responsável pela correspondência
Email: catherine_dmitrieva@outlook.com
Rússia, Krasnodar, 350040
A. Temerdashev
Kuban State University
Email: catherine_dmitrieva@outlook.com
Rússia, Krasnodar, 350040
A. Azaryan
Kuban State University
Email: catherine_dmitrieva@outlook.com
Rússia, Krasnodar, 350040
E. Gashimova
Kuban State University
Email: catherine_dmitrieva@outlook.com
Rússia, Krasnodar, 350040