Chemiliminescence determination of lipid hydroperoxides in biological fluids
- Authors: Proskurnina E.V.1, Dzhatdoeva A.A.1, Lobichenko E.N.1, Shalina R.I.2, Vladimirov Y.A.1
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Affiliations:
- Department of Fundamental Medicine
- Department of Pediatrics
- Issue: Vol 72, No 7 (2017)
- Pages: 751-755
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182548
- DOI: https://doi.org/10.1134/S1061934817050094
- ID: 182548
Cite item
Abstract
To evaluate the oxidative stress under clinical conditions, a procedure is developed for the chemiluminescence determination of the total concentration of lipid hydroperoxides based on their oxidation in the presence of microperoxidase and chemiluminescence activator isoluminol in a borate buffer solution (pH 10.0). The limit of detection for linoleic acid hydroperoxide is 16 nM. The procedure is used to determine lipid hydroperoxides in follicular fluid lipoproteins and in plasma of patients undergoing extracorporeal fertilization.
About the authors
E. V. Proskurnina
Department of Fundamental Medicine
Author for correspondence.
Email: proskurnina@gmail.com
Russian Federation, Moscow, 119192
A. A. Dzhatdoeva
Department of Fundamental Medicine
Email: proskurnina@gmail.com
Russian Federation, Moscow, 119192
E. N. Lobichenko
Department of Fundamental Medicine
Email: proskurnina@gmail.com
Russian Federation, Moscow, 119192
R. I. Shalina
Department of Pediatrics
Email: proskurnina@gmail.com
Russian Federation, Moscow, 117197
Yu. A. Vladimirov
Department of Fundamental Medicine
Email: proskurnina@gmail.com
Russian Federation, Moscow, 119192