Chemiliminescence determination of lipid hydroperoxides in biological fluids
- 作者: Proskurnina E.1, Dzhatdoeva A.1, Lobichenko E.1, Shalina R.2, Vladimirov Y.1
-
隶属关系:
- Department of Fundamental Medicine
- Department of Pediatrics
- 期: 卷 72, 编号 7 (2017)
- 页面: 751-755
- 栏目: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182548
- DOI: https://doi.org/10.1134/S1061934817050094
- ID: 182548
如何引用文章
详细
To evaluate the oxidative stress under clinical conditions, a procedure is developed for the chemiluminescence determination of the total concentration of lipid hydroperoxides based on their oxidation in the presence of microperoxidase and chemiluminescence activator isoluminol in a borate buffer solution (pH 10.0). The limit of detection for linoleic acid hydroperoxide is 16 nM. The procedure is used to determine lipid hydroperoxides in follicular fluid lipoproteins and in plasma of patients undergoing extracorporeal fertilization.
作者简介
E. Proskurnina
Department of Fundamental Medicine
编辑信件的主要联系方式.
Email: proskurnina@gmail.com
俄罗斯联邦, Moscow, 119192
A. Dzhatdoeva
Department of Fundamental Medicine
Email: proskurnina@gmail.com
俄罗斯联邦, Moscow, 119192
E. Lobichenko
Department of Fundamental Medicine
Email: proskurnina@gmail.com
俄罗斯联邦, Moscow, 119192
R. Shalina
Department of Pediatrics
Email: proskurnina@gmail.com
俄罗斯联邦, Moscow, 117197
Yu. Vladimirov
Department of Fundamental Medicine
Email: proskurnina@gmail.com
俄罗斯联邦, Moscow, 119192
![](/img/style/loading.gif)