Enzyme immunoassay for detection of Sudan I dye and its application to the control of foodstuffs
- Authors: Zvereva E.A.1, Zaichik B.T.1, Eremin S.A.2, Zherdev A.V.1, Dzantiev B.B.1
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Affiliations:
- Bakh Institute of Biochemistry
- Department of Chemistry
- Issue: Vol 71, No 9 (2016)
- Pages: 944-948
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182076
- DOI: https://doi.org/10.1134/S1061934816090185
- ID: 182076
Cite item
Abstract
Food safety measures should include the control of the illegal use of banned toxic dyes, including Sudan I as one of the most commonly used coloring agents. A procedure was developed for the enzyme immunoassay of Sudan I carried out in the kinetic mode in order to enhance the performance and rapidity of assays. The achieved limit of detection of Sudan I is 0.13 ng/mL, and the detectable concentration range is from 0.24 to 2.32 ng/mL. The assay time is 50 min. The results obtained by enzyme immunoassay and HPLC for food extracts (tomato paste, chili sauce, spices) showed good correlation. Due to high performance, the new procedure holds promise for the screening control of Sudan I in foodstuffs.
About the authors
E. A. Zvereva
Bakh Institute of Biochemistry
Email: dzantiev@inbi.ras.ru
Russian Federation, Leninskii pr. 33, Moscow, 119071
B. Ts. Zaichik
Bakh Institute of Biochemistry
Email: dzantiev@inbi.ras.ru
Russian Federation, Leninskii pr. 33, Moscow, 119071
S. A. Eremin
Department of Chemistry
Email: dzantiev@inbi.ras.ru
Russian Federation, Moscow, 119991
A. V. Zherdev
Bakh Institute of Biochemistry
Email: dzantiev@inbi.ras.ru
Russian Federation, Leninskii pr. 33, Moscow, 119071
B. B. Dzantiev
Bakh Institute of Biochemistry
Author for correspondence.
Email: dzantiev@inbi.ras.ru
Russian Federation, Leninskii pr. 33, Moscow, 119071