Enzyme immunoassay for detection of Sudan I dye and its application to the control of foodstuffs

Abstract

Food safety measures should include the control of the illegal use of banned toxic dyes, including Sudan I as one of the most commonly used coloring agents. A procedure was developed for the enzyme immunoassay of Sudan I carried out in the kinetic mode in order to enhance the performance and rapidity of assays. The achieved limit of detection of Sudan I is 0.13 ng/mL, and the detectable concentration range is from 0.24 to 2.32 ng/mL. The assay time is 50 min. The results obtained by enzyme immunoassay and HPLC for food extracts (tomato paste, chili sauce, spices) showed good correlation. Due to high performance, the new procedure holds promise for the screening control of Sudan I in foodstuffs.