Email this article Optimization of the Conditions of Analysis of Exhaled Air by Gas Chromatography–Mass Spectrometry for the Noninvasive Diagnostics of Lung Cancer
- Authors: Gorbunov I.S.1, Gubal’ A.R.1, Ganeev A.A.1,2, Rodinkov O.V.1, Kartsova L.A.1, Bessonova E.A.1, Arsen’ev A.I.3, Nefedov A.O.3, Kraeva L.A.4
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Affiliations:
- Institute of Chemistry, St. Petersburg State University
- Institute of Toxicology, Federal Medical-Biological Agency
- Petrov National Oncology Medical Research Center, Ministry of Health of the Russian Federation
- Pasteur Research Institute of Epidemiology and Microbiology
- Issue: Vol 74, No 11 (2019)
- Pages: 1148-1158
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183343
- DOI: https://doi.org/10.1134/S1061934819110042
- ID: 183343
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Abstract
We optimized procedures for the collection, preparation, and storage of samples of exhaled air and the conditions for determining volatile organic compounds in exhaled air by gas chromatography–mass spectrometry (GC–MS), the presence or change in the concentration of which can be caused by lung cancer. The analysis circuit includes group adsorption preconcentration followed by the thermal desorption and determination of analytes by GC–MS. Polymer bags of a Tedlar® film were used for sample collection. The conditions and periods of the storage of samples in bags and adsorption tubes are determined. A method for cleaning and conditioning bags is proposed. For preconcentrating exhaled air components, we used hydrophobic adsorbents (Porapak™ P, Porapak™ Q, and Tenax® TA) and three-section tubes containing Tenax® GR, Carbopack™ B, and Carbosieve® S-III. Their relative efficiency was evaluated by assessing the residual background of gas emission from the adsorbent, the magnitude of the analytical signal, the efficiency of adsorption/desorption, and the efficiency of storage. The conditions for the preconcentration of volatile organic compounds (volume and rate of aspiration, time, and temperature of thermal desorption) are optimized. The main volatile organic compounds in the exhaled air of conditionally healthy volunteers were identified, and their concentrations are evaluated.
About the authors
I. S. Gorbunov
Institute of Chemistry, St. Petersburg State University
Author for correspondence.
Email: i.s.gorbunov@spbu.ru
Russian Federation, St. Petersburg, 198504
A. R. Gubal’
Institute of Chemistry, St. Petersburg State University
Email: i.s.gorbunov@spbu.ru
Russian Federation, St. Petersburg, 198504
A. A. Ganeev
Institute of Chemistry, St. Petersburg State University; Institute of Toxicology, Federal Medical-Biological Agency
Email: i.s.gorbunov@spbu.ru
Russian Federation, St. Petersburg, 198504; St. Petersburg, 192019
O. V. Rodinkov
Institute of Chemistry, St. Petersburg State University
Email: i.s.gorbunov@spbu.ru
Russian Federation, St. Petersburg, 198504
L. A. Kartsova
Institute of Chemistry, St. Petersburg State University
Email: i.s.gorbunov@spbu.ru
Russian Federation, St. Petersburg, 198504
E. A. Bessonova
Institute of Chemistry, St. Petersburg State University
Email: i.s.gorbunov@spbu.ru
Russian Federation, St. Petersburg, 198504
A. I. Arsen’ev
Petrov National Oncology Medical Research Center, Ministry of Health of the Russian Federation
Email: i.s.gorbunov@spbu.ru
Russian Federation, St. Petersburg, 197758
A. O. Nefedov
Petrov National Oncology Medical Research Center, Ministry of Health of the Russian Federation
Email: i.s.gorbunov@spbu.ru
Russian Federation, St. Petersburg, 197758
L. A. Kraeva
Pasteur Research Institute of Epidemiology and Microbiology
Email: i.s.gorbunov@spbu.ru
Russian Federation, St. Petersburg, 197101
