Matrix Interference in the Determination of Elements in Biological Samples by Inductively Coupled Plasma–Mass Spectrometry and Methods for Its Elimination
- Authors: Seregina I.F.1, Osipov K.2, Bol’shov M.A.1,3, Filatova D.G.1, Lanskaya S.Y.1
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Affiliations:
- Department of Chemistry, Moscow State University
- Moscow Institute of Physics and Technology
- Institute of Spectroscopy, Russian Academy of Sciences
- Issue: Vol 74, No 2 (2019)
- Pages: 182-191
- Section: Reviews
- URL: https://journals.rcsi.science/1061-9348/article/view/183155
- DOI: https://doi.org/10.1134/S1061934819020114
- ID: 183155
Cite item
Abstract
The problems of detecting and accounting for spectral and nonspectral interferences arising in the determination of a wide range of elements in biological and pharmaceutical samples by inductively coupled plasma–mass spectrometry are discussed. We studied the features of the effect of matrix components on the accuracy of the results of analysis of biological fluids and pharmaceuticals using a quadrupole mass spectrometer. The processes that have the most significant effect on the suppression of an analytical signal measured by an Agilent 7500c quadrupole mass spectrometer are revealed. The main operational parameters responsible for minimizing the investigated nonspectral interference are determined. Various versions of the internal standard method are studied, which offer a decrease in the effect of the sample composition on the determination results. A direct relationship is found between various modes of operation of the instrument and the criteria for selecting an internal standard. Combined approaches to the complete elimination of nonspectral interference using external calibration are proposed. A method is proposed for decreasing the acidity of the test solution by adsorption separation of the analyte elements and acid.
About the authors
I. F. Seregina
Department of Chemistry, Moscow State University
Author for correspondence.
Email: sereginairinaf@mail.ru
Russian Federation, Moscow, 119991
K. Osipov
Moscow Institute of Physics and Technology
Email: sereginairinaf@mail.ru
Russian Federation, Dolgoprudnyi, Moscow oblast, 141701
M. A. Bol’shov
Department of Chemistry, Moscow State University; Institute of Spectroscopy, Russian Academy of Sciences
Email: sereginairinaf@mail.ru
Russian Federation, Moscow, 119991; Troitsk, Moscow oblast, 108840
D. G. Filatova
Department of Chemistry, Moscow State University
Email: sereginairinaf@mail.ru
Russian Federation, Moscow, 119991
S. Yu. Lanskaya
Department of Chemistry, Moscow State University
Email: sereginairinaf@mail.ru
Russian Federation, Moscow, 119991