Email this article Detection of Ricin in Plant Extracts and Soil Using Liquid Chromatography–High-Resolution Mass Spectrometry
- Authors: Braun A.V.1, Taranchenko V.F.2, Tikhomirov L.A.1, Grechukhin A.P.1, Rybal’chenko I.V.1,2
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Affiliations:
- Kostroma State University
- “27 Scientific Center,” Ministry of Defense of the Russian Federation
- Issue: Vol 73, No 8 (2018)
- Pages: 786-795
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182788
- DOI: https://doi.org/10.1134/S1061934818080026
- ID: 182788
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Abstract
A procedure is developed for the detection of ricin in plant extracts and soil based on its hydrolysis with trypsin and chymotrypsin with the formation of specific peptides and their determination by HPLC with high-resolution tandem mass spectrometric detection. The conditions for the sample preparation of plant extracts and soil samples, mass spectrometric detection in the mode of selected reaction monitoring and gradient elution of specific peptides are optimized. The limit of detection of ricin in extracts is 5 μg/mL. The developed procedure is tested on real samples of plant extracts containing natural ricin.
About the authors
A. V. Braun
Kostroma State University
Author for correspondence.
Email: avbraun@yandex.ru
Russian Federation, Kostroma, 156005
V. F. Taranchenko
“27 Scientific Center,” Ministry of Defense of the Russian Federation
Email: avbraun@yandex.ru
Russian Federation, Moscow, 105005
L. A. Tikhomirov
Kostroma State University
Email: avbraun@yandex.ru
Russian Federation, Kostroma, 156005
A. P. Grechukhin
Kostroma State University
Email: avbraun@yandex.ru
Russian Federation, Kostroma, 156005
I. V. Rybal’chenko
Kostroma State University; “27 Scientific Center,” Ministry of Defense of the Russian Federation
Email: avbraun@yandex.ru
Russian Federation, Kostroma, 156005; Moscow, 105005
