Detection of Ricin in Plant Extracts and Soil Using Liquid Chromatography–High-Resolution Mass Spectrometry
- 作者: Braun A.1, Taranchenko V.2, Tikhomirov L.1, Grechukhin A.1, Rybal’chenko I.1,2
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隶属关系:
- Kostroma State University
- “27 Scientific Center,” Ministry of Defense of the Russian Federation
- 期: 卷 73, 编号 8 (2018)
- 页面: 786-795
- 栏目: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182788
- DOI: https://doi.org/10.1134/S1061934818080026
- ID: 182788
如何引用文章
详细
A procedure is developed for the detection of ricin in plant extracts and soil based on its hydrolysis with trypsin and chymotrypsin with the formation of specific peptides and their determination by HPLC with high-resolution tandem mass spectrometric detection. The conditions for the sample preparation of plant extracts and soil samples, mass spectrometric detection in the mode of selected reaction monitoring and gradient elution of specific peptides are optimized. The limit of detection of ricin in extracts is 5 μg/mL. The developed procedure is tested on real samples of plant extracts containing natural ricin.
作者简介
A. Braun
Kostroma State University
编辑信件的主要联系方式.
Email: avbraun@yandex.ru
俄罗斯联邦, Kostroma, 156005
V. Taranchenko
“27 Scientific Center,” Ministry of Defense of the Russian Federation
Email: avbraun@yandex.ru
俄罗斯联邦, Moscow, 105005
L. Tikhomirov
Kostroma State University
Email: avbraun@yandex.ru
俄罗斯联邦, Kostroma, 156005
A. Grechukhin
Kostroma State University
Email: avbraun@yandex.ru
俄罗斯联邦, Kostroma, 156005
I. Rybal’chenko
Kostroma State University; “27 Scientific Center,” Ministry of Defense of the Russian Federation
Email: avbraun@yandex.ru
俄罗斯联邦, Kostroma, 156005; Moscow, 105005
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