Determination of novobiocin by a kinetic spectrophotometric method in milk and biological samples
- Authors: Keyvanfard M.1, Alizad K.1, Najjarian N.2
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Affiliations:
- Department of Chemistry, Majlesi Branch
- Department of Chemistry, Shahreza Branch
- Issue: Vol 72, No 10 (2017)
- Pages: 1028-1033
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182638
- DOI: https://doi.org/10.1134/S1061934817100124
- ID: 182638
Cite item
Abstract
A new, simple, inexpensive, and fast kinetic spectrophotometric method was developed for the determination of trace amounts of antibiotic drug novobiocin sodium over the range of 4‒32 ng/mL. The method is based on the inhibitory effect of novobiocinon the reaction of Amido Black and periodate in the acidic micellar mediums. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance at 627 nm and fixed-time 6 min from initiation of the reaction. The detection limit is 3 ng/mL, and relative standard deviation for 4.8 and 12 ng/mL novobiocin were 0.52 and 0.65%, respectively, for six replicate measurements. The method was applied to the determination of novobiocin in human and dog serum, urine, and milk samples.
About the authors
Mohsen Keyvanfard
Department of Chemistry, Majlesi Branch
Author for correspondence.
Email: keyvan45638@yahoo.com
Iran, Islamic Republic of, Isfahan
Khadijeh Alizad
Department of Chemistry, Majlesi Branch
Email: keyvan45638@yahoo.com
Iran, Islamic Republic of, Isfahan
Nazila Najjarian
Department of Chemistry, Shahreza Branch
Email: keyvan45638@yahoo.com
Iran, Islamic Republic of, Isfahan