Determination of novobiocin by a kinetic spectrophotometric method in milk and biological samples
- Авторы: Keyvanfard M.1, Alizad K.1, Najjarian N.2
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Учреждения:
- Department of Chemistry, Majlesi Branch
- Department of Chemistry, Shahreza Branch
- Выпуск: Том 72, № 10 (2017)
- Страницы: 1028-1033
- Раздел: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182638
- DOI: https://doi.org/10.1134/S1061934817100124
- ID: 182638
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Аннотация
A new, simple, inexpensive, and fast kinetic spectrophotometric method was developed for the determination of trace amounts of antibiotic drug novobiocin sodium over the range of 4‒32 ng/mL. The method is based on the inhibitory effect of novobiocinon the reaction of Amido Black and periodate in the acidic micellar mediums. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance at 627 nm and fixed-time 6 min from initiation of the reaction. The detection limit is 3 ng/mL, and relative standard deviation for 4.8 and 12 ng/mL novobiocin were 0.52 and 0.65%, respectively, for six replicate measurements. The method was applied to the determination of novobiocin in human and dog serum, urine, and milk samples.
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Mohsen Keyvanfard
Department of Chemistry, Majlesi Branch
Автор, ответственный за переписку.
Email: keyvan45638@yahoo.com
Иран, Isfahan
Khadijeh Alizad
Department of Chemistry, Majlesi Branch
Email: keyvan45638@yahoo.com
Иран, Isfahan
Nazila Najjarian
Department of Chemistry, Shahreza Branch
Email: keyvan45638@yahoo.com
Иран, Isfahan