Use of high-performance liquid chromatography coupled with high-resolution mass spectrometry for the identification and quantitative determination of tetrodotoxin in pharmaceuticals

Abstract

A procedure is developed for the identification and determination of tetrodotoxin in pharmaceuticals by liquid chromatography coupled with high resolution mass spectrometry (HPLC−HRMS). The components of a pharmaceutical sample were separated in the gradient elution mode on a HILIC hydrophilic sorbent and detected by HRMS with electrospray ionization in the mode of registration of selected ion transitions. The calibration curve is linear in the range 0.75−1.25 μg/mL with the correlation coefficient > 0.9995.