An electrochemical aptasensor for cytochrome C, based on pillar[5]arene modified with Neutral Red
- Authors: Stepanova V.B.1, Shurpik D.N.1, Evtyugin V.G.2, Stoikov I.I.1, Evtyugin G.A.1, Gianik T.1,3
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Affiliations:
- Butlerov Institute of Chemistry
- Interdisciplinary Center for Analytical Microscopy
- Comenius University in Bratislava
- Issue: Vol 72, No 4 (2017)
- Pages: 375-381
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182373
- DOI: https://doi.org/10.1134/S1061934817040141
- ID: 182373
Cite item
Abstract
An electrochemical aptasensor is developed for the highly sensitive determination of cytochrome C, using a change in the redox current of Neutral Red covalently bound to terminal carboxyl groups of decasubstituted pillar[5]arene as a signal. The inclusion of the analyte into the complex with an aptamer reduces peaks of redox current of the dye through the dissociation of electron transfer chain in the surface layer. The aptasensor enables the determination of 1 nM to 1.0 mM of cytochrome C in the presence of 1000-fold excesses of albumin, polyethylene glycol, and lysozyme as models of interfering components in biological fluids.
About the authors
V. B. Stepanova
Butlerov Institute of Chemistry
Email: Gennady.Evtugyn@kpfu.ru
Russian Federation, Kazan, 420008
D. N. Shurpik
Butlerov Institute of Chemistry
Email: Gennady.Evtugyn@kpfu.ru
Russian Federation, Kazan, 420008
V. G. Evtyugin
Interdisciplinary Center for Analytical Microscopy
Email: Gennady.Evtugyn@kpfu.ru
Russian Federation, Kazan, 420008
I. I. Stoikov
Butlerov Institute of Chemistry
Email: Gennady.Evtugyn@kpfu.ru
Russian Federation, Kazan, 420008
G. A. Evtyugin
Butlerov Institute of Chemistry
Author for correspondence.
Email: Gennady.Evtugyn@kpfu.ru
Russian Federation, Kazan, 420008
T. Gianik
Butlerov Institute of Chemistry; Comenius University in Bratislava
Email: Gennady.Evtugyn@kpfu.ru
Russian Federation, Kazan, 420008; Bratislava, 842 48
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