Research of the Expression of IL-13 Receptors and IL-13R Genes in the Bronch of Rats with Ovalbumin-Induced Bronchial Asthma

L. E. Blazhevich; Блажевич Л. Е.; O. E. Smirnova; Смирнова О. Е.; V. M. Kirilina; Кирилина В. М.; P. M. Maslyukov; Маслюков П. М.

doi:10.31857/S0869813923040039

Research of the Expression of IL-13 Receptors and IL-13R Genes in the Bronch of Rats with Ovalbumin-Induced Bronchial Asthma

作者: Blazhevich L.¹, Smirnova O.¹, Kirilina V.¹, Maslyukov P.²
隶属关系:
1. Petrozavodsk State University
2. Yaroslavl State Medical University of the Ministry of Healthcare of the Russian Federation
期: 卷 109, 编号 4 (2023)
页面: 530-544
栏目: ЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИ
URL: https://journals.rcsi.science/0869-8139/article/view/137930
DOI: https://doi.org/10.31857/S0869813923040039
EDN: https://elibrary.ru/VIWGPC
ID: 137930

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详细

This article presents the results of studies of the levels of expression of the IL13R gene and the levels of expression of the molecular IL13R in the bronchi of control rats and rats with ovalbumin-induced asthma. The choice of these receptors is due to the great importance of these structures in the pathogenesis of allergic bronchial asthma. Biological materials of 64 Wistar rats were studied using real-time PCR and immunohistochemical methods. As a result of the studies, it was found that under conditions of asthma development in the tissues of the bronchi of rats, a pronounced expression of the IL13R genes, a significant expression of the molecular receptor for interleukin-13, occurs. In bronchi with an intramural ganglion (bifurcation zone), the level of IL13R gene expression and the level of expression of the IL13R molecular receptor were significantly higher than in tracheal samples without ganglia. Sodium cromoglycate, administered to rats 5 hours after the last inhalation of ovalbumin, led to a decrease in the mRNA content in the bronchi of animals compared to animals that did not receive mast cell stabilizer treatment.

关键词

: interleukin-13, model of ovalbumin-induced bronchial asthma, intramural ganglion, sodium cromoglycate

参考

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