Electron microscopy of stable electrophoretic fractions of natural humic acids – the key to the understanding of their structural organization
- Autores: Trubetskaya O.1, Selivanova O.2, Rogachevsky V.3, Trubetskoj O.4
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Afiliações:
- Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS
- Institute of Protein Research, Russian Academy of Sciences
- Institute of Cell Biophysics, Russian Academy of Sciences
- Institute of Basic Biological Problems, Russian Academy of Sciences
- Edição: Volume 50, Nº 3 (2024)
- Páginas: 324-334
- Seção: Articles
- URL: https://journals.rcsi.science/0132-3423/article/view/261485
- DOI: https://doi.org/10.31857/S0132342324030118
- EDN: https://elibrary.ru/NYSDGM
- ID: 261485
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Resumo
Transmission electron microscopy (TEM) with contrast staining by uranyl acetate solution was used to study morphological differences between soil humic acids (HAs) and their A, B and C + D fractions obtained using coupling preparative low-pressure size exclusion chromatography and analytical polyacrylamide gel electrophoresis. The electrophoretic mobility of fractions varied in order C + D > B > A. The distribution of various morphological elements between fractions showed that large structures such as vesicle-like formations 70–150 nm long and 30–80 nm wide with clear edges were found exclusively in fraction A and occupied ~55% of the TEM image area. On the other hand, long fibrils with a length of 60–100 nm, a width of 4–6 nm and a thickness of 2–3 nm, as well as their bundles with a length of >150 nm and a diameter of 30–70 nm were identified only in the C + D fraction and occupied ~59 % area of TEM images. Smaller morphological elements such as point particles with a diameter of 2–3 nm, ring particles with a diameter of 4–6 nm, worm-shaped short particles with a length of 20–30 nm, and spheroids with a diameter of 10– 30 nm were observed in all samples, but in varying quantities. Significant morphological differences between the fractions can be explained by their composition, previously established by using a few physico-chemical methods. The ratio Car(165–108 ppm)/Calk(108–0 ppm), or aromaticity index, calculated from 13C-NMR, could be one of the indicators of the various morphological structures formation. The obtained TEM results clearly confirm the supramolecular organization of soil HAs.
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Sobre autores
O. Trubetskaya
Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS
Autor responsável pela correspondência
Email: olegi03@yahoo.com
Rússia, prosp. Nauki, 6, Pushchino, 142290
O. Selivanova
Institute of Protein Research, Russian Academy of Sciences
Email: olegi03@yahoo.com
Rússia, prosp. Nauki, 4, Pushchino, 142290
V. Rogachevsky
Institute of Cell Biophysics, Russian Academy of Sciences
Email: olegi03@yahoo.com
Rússia, prosp. Nauki, 3, Pushchino, 142290
O. Trubetskoj
Institute of Basic Biological Problems, Russian Academy of Sciences
Email: olegi03@yahoo.com
Rússia, prosp. Nauki, 2, Pushchino, 142290
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