The 3.6 Å cryo-em structure of the outer heptameric α-ring of human 26s immunoproteasome in the preactivation state
- Autores: Saratov G.1, Baymukhametov T.2, Konevega A.2,3,4, Kudriaeva А.1, Belogurov А.1,5
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Afiliações:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
- National Research Center, “Kurchatov Institute”
- Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre, Kurchatov Institute
- Institute of Biomedical Systems and Biotechnologies, Peter the Great St. Petersburg Polytechnic University
- Evdokimov Moscow State University of Medicine and Dentistry
- Edição: Volume 50, Nº 3 (2024)
- Páginas: 311-323
- Seção: Articles
- URL: https://journals.rcsi.science/0132-3423/article/view/261484
- DOI: https://doi.org/10.31857/S0132342324030101
- EDN: https://elibrary.ru/NYZKEM
- ID: 261484
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Resumo
The 26S proteasome is a unique multicatalytic proteinase complex, together with a ubiquitination system, providing controlled degradation of most intracellular eukaryotic proteins. The problem of studying the proteasome is the multiplicity of its intracellular forms, which are formed due to the modularity of the proteasome assembly process. In this study, using cryoelectron microscopy, we described for the first time the structure of the 26S human immunoproteasome in comparison with its constitutive form with a resolution of 3.6 Å. A detailed analysis of the structural features of the two complexes revealed the opening of the entrance in the outer heptameric 20S ring of the immunoproteasome subunit due to the separation of the N-terminal regions of the PSMA4 and PSMA5 subunits and the formation of a π–π stacking between the amino acid residues Tyr5 and Phe9 of the PSMA5 and PSMA6 subunits, respectively. The revealed removal of steric obstruction in the central channel of the 20S subunit may indicate the preactivation phenotype of the 26S human immunoproteasome, even in the absence of a bound substrate.
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Sobre autores
G. Saratov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
Email: belogurov@ibch.ru
Rússia, ul. Miklukho-Maklaya 16/10, Moscow, 117997
T. Baymukhametov
National Research Center, “Kurchatov Institute”
Email: belogurov@ibch.ru
Rússia, pl. Academica Kurchatova 1, Moscow, 123182
A. Konevega
National Research Center, “Kurchatov Institute”; Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre, Kurchatov Institute; Institute of Biomedical Systems and Biotechnologies, Peter the Great St. Petersburg Polytechnic University
Email: belogurov@ibch.ru
Rússia, pl. Academica Kurchatova 1, Moscow, 123182; mcr. Orlova Roshcha 1, Gatchina, 188300; ul. Politekhnicheskaya 29, St. Petersburg, 195251
А. Kudriaeva
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS
Email: belogurov@ibch.ru
Rússia, ul. Miklukho-Maklaya 16/10, Moscow, 117997
А. Belogurov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS; Evdokimov Moscow State University of Medicine and Dentistry
Autor responsável pela correspondência
Email: belogurov@ibch.ru
Rússia, ul. Miklukho-Maklaya 16/10, Moscow, 117997; ul. Delegatskaya 20/1, Moscow, 127473
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