Synthesis of bifunctional lipophilic constructs
- Authors: Anisimova D.O.1, Savchenko M.S.1, Tuzikov A.B.1, Paramonov A.S.1, Chizhov A.O.2, Bovin N.V.1, Ryzhov I.M.1
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Affiliations:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- N. D. Zelinsky Institute of Organic Chemistry of the Russian Academy of Sciences
- Issue: Vol 50, No 3 (2024)
- Pages: 256-268
- Section: Articles
- URL: https://journals.rcsi.science/0132-3423/article/view/261464
- DOI: https://doi.org/10.31857/S0132342324030046
- EDN: https://elibrary.ru/OABYOT
- ID: 261464
Cite item
Abstract
An ability of glycolipids to embed membrane of living cells opens an opportunity to modify cellular surface via insertion of synthetic lipophilic constructs carrying given glycan (or any other molecular fragment). Detection of thus inserted glycans by fluorescent microscopy requires treatment with corresponding fluorescently labeled antibodies. Di- (IgG) and decavalent (IgM) antibodies can significantly affect the distribution of glycolipids in the membrane, therefore direct visualization of embedded lipophilic constructs is required. To achieve this, fluorescent tag must be included in the composition of the lipophilic constructs and at the same time be located at a sufficient distance from glycan part. Here we propose two approaches to the synthesis of these compounds and describe obtaining of two constructs carrying A (type 2) tetrasaccharide and either fluorescein or sulfo-cyanine-3.
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About the authors
D. O. Anisimova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: imryzhov@gmail.com
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
M. S. Savchenko
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: imryzhov@gmail.com
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
A. B. Tuzikov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: imryzhov@gmail.com
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
A. S. Paramonov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: imryzhov@gmail.com
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
A. O. Chizhov
N. D. Zelinsky Institute of Organic Chemistry of the Russian Academy of Sciences
Email: imryzhov@gmail.com
Russian Federation, Leninskiy prosp. 47, Moscow, 119991
N. V. Bovin
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: imryzhov@gmail.com
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
I. M. Ryzhov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Author for correspondence.
Email: imryzhov@gmail.com
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
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