Immobilization of Azospirillum Bacteria on Various Carriers

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Abstract

A significant part of research in environmentally friendly agroindustrial production is aimed at immobilized bacterial preparations with retained capacity for active growth without loss of metabolic activity both during immobilization and after long-term storage and biotechnological use. In the present work, immobilization of members of the genus Azospirillum on natural and synthetic carriers was investigated. Efficiency of immobilization of the cells of A. brasilense strain SR80 in alginate hydrogel and vermiculite was investigated. Proliferative and metabolic activities of immobilized preparations were investigated. The prospects of using vermiculite and calcium alginate as matrices for Azospirillum immobilization are shown.

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About the authors

M. A. Kupryashina

Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences; Saratov State Medical University named after V. I. Razumovsky

Author for correspondence.
Email: kupryashina_m@mail.ru
Russian Federation, Saratov; Saratov

E. G. Ponomareva

Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences

Email: kupryashina_m@mail.ru
Russian Federation, Saratov

T. E. Pylaev

Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences; Saratov State Medical University named after V. I. Razumovsky

Email: kupryashina_m@mail.ru
Russian Federation, Saratov; Saratov

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Supplementary files

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2. Fig. 1. Assessment of viability of Azospirillum cells immobilized on different carriers when sown on dense nutrient medium: a - Ca-alginate beads; b - vermiculite; c - silica gel

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3. Fig. 2. Relative respiratory activity of bacterial suspension (a - calibration curve) and immobilized bacteria (b) on three types of carriers: alginate beads, vermiculite and silica gel. The lower threshold was determined by the baseline (signal/noise). The respiratory activity of bacterial suspension with OP600 = 0 was taken as 100%

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4. Fig. 3. SEM images of Ca-alginate beads (a), vermiculite (b) and silica gel (c) immobilized by Azospirillum cells

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5. Fig. 4. SEM images of the structure of Ca-alginate beads (a), vermiculite (b) and silica gel (c), control (1) and immobilized by Azospirillum cells (2)

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6. Fig. 5. Variation of specific activity of polyphenol oxidase of suspension (1), immobilized on vermiculite (2) and on alginate beads (3) of A. brasilense SR80 cells

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