Combination of Mesenchymal Stromal Cells and Cardiac Stem Cells in a Multilayer Cell Construct Promotes Activation of Notch Signaling and Initiation of Endothelial Differentiation
- Autores: Shevchenko E.1, Dergilev K.1, Tsokolaeva Z.1, Beloglazova I.1, Molokotina Y.1, Parfenova E.1, Men’shikov M.1
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Afiliações:
- National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
- Edição: Volume 166, Nº 4 (2019)
- Páginas: 548-552
- Seção: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/241174
- DOI: https://doi.org/10.1007/s10517-019-04390-7
- ID: 241174
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Resumo
We showed the possibility of generating combined tissue-engineered cell consisting of layers of rat cardiac stem cells and mesenchymal stromal cells from the adipose tissue. Cell—cell interaction within the cell sheet promoted proliferation of cardiac stem cells, expression of endothelial differentiation marker ETS1, and Notch signaling activation. The obtained results provide new insights into possible mechanisms of stimulation of endogenous regeneration processes after myocardial damage and demonstrate potential of cell-based cardiomyoplasty with the use of these combined cell sheets.
Sobre autores
E. Shevchenko
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Autor responsável pela correspondência
Email: dr.eshevchenko@gmail.com
Rússia, Moscow
K. Dergilev
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: dr.eshevchenko@gmail.com
Rússia, Moscow
Z. Tsokolaeva
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: dr.eshevchenko@gmail.com
Rússia, Moscow
I. Beloglazova
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: dr.eshevchenko@gmail.com
Rússia, Moscow
Yu. Molokotina
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: dr.eshevchenko@gmail.com
Rússia, Moscow
E. Parfenova
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: dr.eshevchenko@gmail.com
Rússia, Moscow
M. Men’shikov
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: dr.eshevchenko@gmail.com
Rússia, Moscow