Combination of Mesenchymal Stromal Cells and Cardiac Stem Cells in a Multilayer Cell Construct Promotes Activation of Notch Signaling and Initiation of Endothelial Differentiation
- Authors: Shevchenko E.K.1, Dergilev K.V.1, Tsokolaeva Z.I.1, Beloglazova I.B.1, Molokotina Y.D.1, Parfenova E.V.1, Men’shikov M.Y.1
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Affiliations:
- National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
- Issue: Vol 166, No 4 (2019)
- Pages: 548-552
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/241174
- DOI: https://doi.org/10.1007/s10517-019-04390-7
- ID: 241174
Cite item
Abstract
We showed the possibility of generating combined tissue-engineered cell consisting of layers of rat cardiac stem cells and mesenchymal stromal cells from the adipose tissue. Cell—cell interaction within the cell sheet promoted proliferation of cardiac stem cells, expression of endothelial differentiation marker ETS1, and Notch signaling activation. The obtained results provide new insights into possible mechanisms of stimulation of endogenous regeneration processes after myocardial damage and demonstrate potential of cell-based cardiomyoplasty with the use of these combined cell sheets.
About the authors
E. K. Shevchenko
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Author for correspondence.
Email: dr.eshevchenko@gmail.com
Russian Federation, Moscow
K. V. Dergilev
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: dr.eshevchenko@gmail.com
Russian Federation, Moscow
Z. I. Tsokolaeva
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: dr.eshevchenko@gmail.com
Russian Federation, Moscow
I. B. Beloglazova
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: dr.eshevchenko@gmail.com
Russian Federation, Moscow
Yu. D. Molokotina
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: dr.eshevchenko@gmail.com
Russian Federation, Moscow
E. V. Parfenova
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: dr.eshevchenko@gmail.com
Russian Federation, Moscow
M. Yu. Men’shikov
National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation
Email: dr.eshevchenko@gmail.com
Russian Federation, Moscow