Combined Effect of TLR2 Ligands on ROS Production by Mouse Peritoneal Macrophages
- Autores: Teselkin Y.1, Khoreva M.1, Veselova A.1, Babenkova I.1, Osipov A.1, Gankovskaya L.1, Vladimirov Y.1
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Afiliações:
- N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
- Edição: Volume 166, Nº 1 (2018)
- Páginas: 26-30
- Seção: Biophysics and Biochemistry
- URL: https://journals.rcsi.science/0007-4888/article/view/240806
- DOI: https://doi.org/10.1007/s10517-018-4281-9
- ID: 240806
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Resumo
TLR2-mediated ROS production by mouse peritoneal macrophages was studied by luminoldependent chemiluminescence under conditions of cell stimulation with zymosan (TLR2/6 ligand) and peptidoglycan (TLR2/1 ligand). ROS production by macrophages stimulated with zymosan and peptidoglycan simultaneously depended on the ratio of ligand concentrations. Three effects were revealed: additivity of the stimulating effects of the ligands used, competitive ligand binding, and effect of macrophage priming with peptidoglycan during cell stimulation with zymosan. The mechanisms of these effects are discussed.
Sobre autores
Yu. Teselkin
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Autor responsável pela correspondência
Email: teselkin-box@mail.ru
Rússia, Moscow
M. Khoreva
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: teselkin-box@mail.ru
Rússia, Moscow
A. Veselova
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: teselkin-box@mail.ru
Rússia, Moscow
I. Babenkova
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: teselkin-box@mail.ru
Rússia, Moscow
A. Osipov
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: teselkin-box@mail.ru
Rússia, Moscow
L. Gankovskaya
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: teselkin-box@mail.ru
Rússia, Moscow
Yu. Vladimirov
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: teselkin-box@mail.ru
Rússia, Moscow