Combined Effect of TLR2 Ligands on ROS Production by Mouse Peritoneal Macrophages
- Authors: Teselkin Y.O.1, Khoreva M.V.1, Veselova A.V.1, Babenkova I.V.1, Osipov A.N.1, Gankovskaya L.V.1, Vladimirov Y.A.1
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Affiliations:
- N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
- Issue: Vol 166, No 1 (2018)
- Pages: 26-30
- Section: Biophysics and Biochemistry
- URL: https://journals.rcsi.science/0007-4888/article/view/240806
- DOI: https://doi.org/10.1007/s10517-018-4281-9
- ID: 240806
Cite item
Abstract
TLR2-mediated ROS production by mouse peritoneal macrophages was studied by luminoldependent chemiluminescence under conditions of cell stimulation with zymosan (TLR2/6 ligand) and peptidoglycan (TLR2/1 ligand). ROS production by macrophages stimulated with zymosan and peptidoglycan simultaneously depended on the ratio of ligand concentrations. Three effects were revealed: additivity of the stimulating effects of the ligands used, competitive ligand binding, and effect of macrophage priming with peptidoglycan during cell stimulation with zymosan. The mechanisms of these effects are discussed.
About the authors
Yu. O. Teselkin
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Author for correspondence.
Email: teselkin-box@mail.ru
Russian Federation, Moscow
M. V. Khoreva
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: teselkin-box@mail.ru
Russian Federation, Moscow
A. V. Veselova
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: teselkin-box@mail.ru
Russian Federation, Moscow
I. V. Babenkova
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: teselkin-box@mail.ru
Russian Federation, Moscow
A. N. Osipov
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: teselkin-box@mail.ru
Russian Federation, Moscow
L. V. Gankovskaya
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: teselkin-box@mail.ru
Russian Federation, Moscow
Yu. A. Vladimirov
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: teselkin-box@mail.ru
Russian Federation, Moscow