Role of β Cell Precursors in the Regeneration of Insulin-Producing Pancreatic β Cells under the Influence of Glucagon-Like Peptide 1
- Авторлар: Dygai A.1, Skurikhin E.1, Pakhomova A.1, Epanchintsev A.1, Stronin O.1, Ermakova N.1, Pershina O.1, Ermolaeva L.1, Krupin V.1, Kudryashova A.1, Zhdanov V.1
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Мекемелер:
- Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
- Шығарылым: Том 165, № 5 (2018)
- Беттер: 644-648
- Бөлім: Pharmacology and Toxicology
- URL: https://journals.rcsi.science/0007-4888/article/view/240626
- DOI: https://doi.org/10.1007/s10517-018-4232-5
- ID: 240626
Дәйексөз келтіру
Аннотация
The effects of the pegylated form of glucagon-like peptide 1 (pegGLP-1) on oligopotent β cell precursors (CD45—TER119—CD133+CD49flow) in the pancreas were studied in C57Bl/6 mice. Under conditions of streptozotocin-induced type 1 diabetes mellitus, intraperitoneal injection of pegGLP1 increased the content of β cell precursors and dithizone-stained cells in the pancreas. β Cell precursors of mice with diabetes demonstrated high self-maintenance potential. In contrast to pegGLP-1, native GLP-1 did not affect β cell precursors in diabetic animals. Treatment of a culture of β cell precursors from mice with diabetes induced the yield of dithizone-stained mononuclears. In conditioned mediums of dithizone-positive cells obtained as a result of differentiation of β cell precursors from mice with diabetes, insulin was detected after administration of pegGLP-1 (10—7 M) and glucose (3 mmol/liter); the level of insulin increased with increasing glucose concentration (to 20 mmol/liter). The in vitro effect of pegGLP-1 did not differ from the effect of GLP-1 (10—7 M).
Авторлар туралы
A. Dygai
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Ресей, Tomsk
E. Skurikhin
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: eskurikhin@inbox.ru
Ресей, Tomsk
A. Pakhomova
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Ресей, Tomsk
A. Epanchintsev
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Ресей, Tomsk
O. Stronin
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Ресей, Tomsk
N. Ermakova
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Ресей, Tomsk
O. Pershina
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Ресей, Tomsk
L. Ermolaeva
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Ресей, Tomsk
V. Krupin
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Ресей, Tomsk
A. Kudryashova
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Ресей, Tomsk
V. Zhdanov
Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences
Email: eskurikhin@inbox.ru
Ресей, Tomsk
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